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Figure 6
Ahi1 mutations via CRISPR/Cas9 lead to abnormal development of zebrafish. (A) The CRISPR/Cas9 target sites were designed to create mutations in exon 12 and exon 14, which were close to the ahi1 morpholino targeting site. (B) T7E1 assay results showed cleaved ahi1 DNA products by CRISPR/Cas9. (C) Sequencing data showing ahi1 DNA mutations in the ahi1 CRSIPR/Cas9-targeted (ahi1 mutant) embryos. (D) The morphology of WT and ahi1 mutant zebrafish at 3 months.
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Full text @ Front. Cell. Neurosci.