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Fig. 4
Liposomal etomoxir and MitoTEMPO suppress MSU crystal-driven expression of il1b and production of Tnfa by macrophages. (A)Expression of il1b after co-injection of MSU crystals with liposomal PBS (L-PBS), etomoxir (L-Eto.), or MitoTEMPO (L-MT) compared with PBS-only injected controls, as detected at 3 hpi. (B) Temporal quantification of il1bexpression, as detected in A. (C) Immunofluorescence detection of Tnfa within the hindbrain of Tg(mpeg1:EGFP) larvae co-injected with MSU crystals and L-PBS, L-Eto, or L-MT, as detected at 3 hpi. (D) Quantification of Tnfa, as detected in (C) (n = 15 larvae/treatment). Black arrow marks il1b-expressing macrophages in hindbrain. All views anterior to left. Error bars display mean ± SD. ****p < 0.0001, one-way ANOVA, Dunnett's post hoc test. Scale bars, 100 μm in (A) and 50 μm in (C).