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Fig. 5
Ezh1 ul3/ul3 zebrafish mutants present a normal phenotype. (A) Pictures of 6 month-old MZezh1ul3/ul3 mutant female (top) and male (bottom) zebrafish from the F2 generation of incross mutant fish. (B) Global trimethylation (H3K27me3) and demethylation (H3K27me2) of lysine 27 of histone H3 is not affected in ezh1ul3/ul3 mutants. Total histones from pools of 10 larvae at 9 dpf from wild-type or ezh1ul3/ul3 crosses as indicated, were extracted and 5 µg of total histones were analyzed by western blotting using specific anti-H3K27me3 or anti-H3K27Me2 antibodies and an anti-H3 antibody as a control.
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