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Fig. 3
Ezh1 expression during zebrafish development. (A) In situ hybridization at the 2-cell stage, 6 hpf, 24 hpf and 48 hpf showing that ezh1 transcripts are maternally provided (2-cell stage) and showing zygotic ezh1 mRNA distribution during early development. The arrowhead shows the gut and the arrow the pectoral fin bud. Scale bar is 500 μm. (B) RT-PCR analysis showing detection of ezh1 and ezh2 mRNAs at 1 hpf, 6 hpf, 1 dpf, 2 dpf, 3 dpf and 4 dpf. The reverse transcriptase (RT) was included (+) or not (−) in the reaction, as indicated. (C) RT-qPCR showing that relative ezh1 expression increases during larval development from 1 dpf to 5 dpf whereas ezh2 expression decreases. Three independent experiments were performed and error bars represent standard deviation. Statistical analysis was performed using a one-way ANOVA with Tukey’s post hoc test. **P < 0.01; ***P < 0.001.
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