Fig. 2

(A) The positions of TSS-deletion allele and gene deletion allele are marked by dashed red lines. Green box represents the conserved element in cyrano which is complementary to miR-7. Solid red lines indicate the position of the first exon-intron boundary (e1i1) morpholino and conserved microRNA binding site (CMiBS) morpholinos. Arrows flanking black dotted line mark the primer binding sites for qRT-PCR product. (B) Representative images of in situ hybridization for cyrano in wild type (15/15) and both homozygous TSS-deletion (21/22) and gene deletion (18/18) 1-dpf. (C) At 2-dpf gene deletion mutants (lower-left), (and TSS-deletion mutants, not shown) were not different from the wild-type embryos (upper-left). Morpholino injected wild-type embryos (upper-middle and upper-left) reproduced observed phenotype in Ulitsky et. al (Kok et al., 2015). Morpholino injected deletion-mutants, lacking the corresponding binding sites for morpholinos, (lower-middle and lower-left) were comparable to morpholino injected wild types.