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Fig. 7
Timecourse of promoter activity in lens (A) and skeletal muscle (B) in all combined embryos.
Zebrafish αA-crystallin promoter (circles) produced lens expression in a larger proportion of embryos by 36 hpf and drove GFP expression in over 85% of embryos by 72 hpf. The human βB1-crystallin promoter (triangles) drove surprisingly abundant expression in zebrafish skeletal muscle, but was less active in lens. Between 11 and 66 embryos were observed for each timepoint. Images C-F show representative examples of GFP expression with each promoter as indicated in lens (C and D) and skeletal muscle (E and F).
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