ZFIN Image: Lan et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Epicardial Cells from Donor Hearts Do Not Migrate onto tet2/3^DM Recipient Hearts

(A) Schematic of epicardial cell migration assay. Wild-typetcf21:DsRed donor hearts (isolated at 72 hpf) were co-cultured with either wild-type or tet2/3^DM myl7:GFP recipient hearts (isolated at 48 hpf) for one week and then confocal images taken.

(B) Confocal images showed epicardial cells from wild-type donor heart can migrate onto wild-type recipient hearts. In 30 pairs of co-cultured hearts, 6 of recipient hearts were observed having epicardial cells migrated from donor hearts.

(C) Confocal images showed epicardial cells from wild-type donor heart failed to migrate onto tet2/3^DM recipient hearts. In 60 pairs of co-cultured hearts, none of the recipient hearts were observed having epicardial cells from donor hearts.

Scale bar: 50 μm.

Acknowledgments

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