Fig. 6

Immunoreactivity of serotonin was altered in the morphants and mutants at 5 dpf. (A) (a–d) Serotonin immunoreactivity was reduced in the morphant. (a) Ctrl. (b) Ntrk2b MO. (C) Ntrk2a MO and (d) Ntrk2b + mRNA. (B) Total serotonin positive cell counts shows significant reduction in the Ntrk2b MO. (A) (a′–d′) Higher magnification images of the PVOa and (A) (a′′–d′′) Higher magnification images of the PVOp. (C) The Ntrk2b mutants had reduced serotonin immunoreactivity as compared to wild-type littermates (a) wild-type, (b) Mutant, (c) Mutant injected with ntrk2b mRNA. The expression of 5-HT in the mutant is aberrant. (C) (a′–d′) Higher magnification images of the PVOa and (C) (a′′–d′′) Higher magnification images of the PVOp. (D) Comparative cell counts from the three groups representing reduced cell numbers in the mutant. PVOa – paraventricular organ, anterior part, PVOi – paraventricular organ, intermediate part, PVOp – paraventricular organ. (Bars represent Mean ± SEM, one-way ANOVA. **p-value < 0.002, ***p-value < 0.0004). Scale bar = 100 µm.