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Fig. 5
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Fig. 5
RNAscope in situ hybridisation for visualisation of selected differentially expressed gene (DEG) events from RNA-seq transcriptome analysis. Representative images of dual in situ hybridisation in the wild-type zebrafish retina using mRNA probes to (A–C) vax1 and bambia, (D–F) tbx3a and pax2a, and (G–I) rbp2a and pax2a at 32, 48 and 56 hpf. Nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI). White dotted lines indicate the location of the lens. Scale bar 50 µm.
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