|
Fig. 4
Laminin alpha 5 inhibits invasion in a brain microenvironment. A) Representative maximum projection images of 4D U251MG cell tracking in zebrafish larvae brain. Left to right: CM-DiI-labeled U251MG cells and Trackmate calculated tracks where each color represents the 4D coordinate data of individual cells; 100 μm scale bar. B) Histogram of distance traveled for all U251MG cells (lama5 n = 331, control n = 500). C) Percentage of non-invasive U251MG cells for control and lama5 KD larvae. *Fisher's Exact Test, P < 0.001. D-G) Average total distance (D), average median invasion rate (E), average number of times invasion was initiated per hour (F), average number of times invasion was initiated per cell (G), for lama5 (n = 9) and control (n = 8) zebrafish where each point represents the average U251MG cell statistic per larva. P-values determined by Student's T Test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)