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Fig. 3
Phenotypic characterization of intestinal tumorigeneis in kras+ transgenic zebrafish. Wild-type and kras+ fish were treated with 2 or 3 μM mifepristone from 2 mpf, and samples were collected at 2, 4, and 6 wpi for gross observations and histological analyses. (A) Body length. (B) Body weight. (C) Survival curves. (D) Examples of normal, enteritis, hyperplasia, and tubular adenoma from H&E staining sections of intestine. Wild-type or kras+ fish sources of the sections are indicated. (E) Summary of intestinal histological abnormalities observed in wild-type and kras+ transgenic fish. The data were generated as a result of a blinded histological analysis. Numbers of fish in each group are indicated at the top of each bar. The differences among the variables were assessed using Student's t tests or one-way ANOVA. Statistical significance: *P < .05, **P < .01, ***P < .001.