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Fig. 2

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ZDB-IMAGE-190115-10
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Figures for Zhang et al., 2018
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Fig. 2

Generation of mtu1 knock-out zebrafish using CRISPR/Cas9 system. (A) Schematic representation of CRISPR/Cas9 target site at exon 4 as used in this study. An allele, mtu1ins32bp was produced by a 32 bp insertion in exon 4 and a truncated 145 amino acid non-functional protein. (BD) Genotyping of mtu1ins32bp by Sanger sequence, the PAGE RFLP and Western blot analyses. (E) The morphology of mtu1−/−, mtu1+/− and mtu1+/+ zebrafish at 3 dpf. (F) The ratios of genotypes/phenotype of offsprings (F2) in clutches from different F1 mtu1 heterozygous crosses at 10 dpf (n = 350).

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