|
Fig. 5
The Bcl9–Pygo complex acts in neural crest and cardiac heart progenitor cells. (A) Schematic representation of the RNA sequencing (RNA-seq) approach used for bcl9Δ29 mutant zebrafish embryos. RNA extraction was performed on manually dissected anterior structures of 54-hpf embryos as indicated by the dashed box. (B) Comparing three independent biological replicates revealed 157 differentially expressed (74 up-regulated and 83 down-regulated) genes between wild-type and bcl9Δ29 mutants as represented in the volcano plot. Genes associated with cardiac development are highlighted. (C–F) Hematoxylin/eosin staining of heart sections of control (C,E) compared with Wnt1-Cre; Pygo1/2-flox (D) and Bcl9/9l-ΔHD1/flox (F) 13.5-dpc siblings. (G–J) Pax3 expression in 10.5-dpc embryos in the region of branchial arches: view of whole-mount mRNA ISH of control (G) compared with Pygo1/2 knockout (KO; I) siblings. H and J are magnified insets of the regions marked with dashed squares in G and I, respectively. (K–N) Hematoxylin/eosin staining of heart sections of control (K) compared with Nkx2.5-Cre; Bcl9/9l-flox (L–N) 13.5-dpc siblings.