ZFIN Image: Abdul-Wajid et al., 2018, Fig. S8

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Figure Caption

Fig. S8

Jag2b fluorescent in-situ hybridization in NC-Cm labelled embryos. 3dpf hearts from NC-Cm labelled embryos were subject to the fluorescent in-situ protocol with and without jag2b-TRITC probe as well as immunostaining for tRFP and GFP. Remnant RFP expression from the NC-Cms is present in the tritc channel however distinct from jag2b probe detection (white arrows and astericks compared to no-probe controls). White arrows show NC-Cm specific colocalization of jag2b signal and asterisks show other Cm expression or non-Cm expression. The strong tail expression of jag2b was used as a positive control for the fluorescent in-situ hybridization experiment.

Acknowledgments

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