Fig. 5

Fig. 5

Bmp2b mRNA reversed the reduction in the number of olig2⁺ cells migrating to the dorsal area. (A) Bmp2b mRNA was injected into one-cell embryos. (B) qRT-PCR analysis showed that bmp2b gene expression increased, validating the rescue effect. (C–D) bmp2b mRNA reversed the reduction in the number of dorsally migrated olig2⁺ cells at 3 and 4 dpf. A1′, A2′, A3′, and A4′ are the magnified pictures of the white boxes in A1, A2, A3, and A4. Scale bars: A1–A4, 50 μm and A1′–A4′, 10 μm. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001. Error bars represent S.E.M.