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Fig. S4
Upper panel, left. Quantitation by qPCR of gene expression of gata5, nkx2.5, the smarcd3 genes and ntl. 24 hpf embryos were genotyped and evaluated. For all genes n ≥3. No significant fold change was detected. Right. Quantitation by qPCR of gata5 gene expression in isolated hearts of 4 dpf defective embryos compared to hearts of normal siblings indicated a significant difference (P value = 0,0087). Lower panel. Expression of gata5, smarce1, nppa and nkx2.5 in 4 dpf (smarce1) and 5 dpf (all other markers) normal and defective larvae detected by ISH (lateral views).
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