ZFIN Image: Mayr et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Localization of ERK-KTR control constructs T55V/T62V and T55D/T62D. 48 hpf zebrafish embryos expressing either ERK-KTR-mClover or T55V/T62V and T55D/T62D control reporter constructs. (A) Neural cells within the CNS expressing ERK-KTR-mClover. The reporter localizes to the cytoplasm. (B) The T55V/T62V control reporter is also found in the nucleus of neural cells within the CNS. (C) Muscle cells expressing ERK-KTR-mClover. (D) The phosphomimetic T55D/T62D reporter construct localizes predominantly to the cytoplasm when expressed in muscle cells. All scale bars are 25 μm. Images were recorded on a Leica Sp8 X WLL confocal system and rendered with Adobe Photoshop CS6.

Acknowledgments

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