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Fig. 3
Monitoring small compound uptake kinetics in DREKA zebrafish (A) 55 hpf DREKA embryos were embedded in agarose containing 10 μM trametinib to investigate compound uptake kinetics in skin epithelial cells. After 10 min skin epithelial cells still show active Erk signaling. Around 20 min Erk activity is visibly decreased and by 50–60 min cells show full Erk signaling inhibition. (B) Quantification of Erk activity over time. Cytoplasmic to nuclear mClover intensity over time in three representative skin epithelial cells in DREKA zebrafish treated with 10 μM trametinib at 55 hpf. All scale bars are 25 μm. All images were recorded on a Leica SP8 X WLL confocal microscope and rendered using Photoshop CS6.