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Fig. S3
Comparison of Rb1 expression in the F0 embryo injection melanoma (n=1) versus the TEAZ melanoma (n=1). On the left is a melanoma created by injection of an mitfa:BRAF-tdTomato(fusion) transgene into a p53-/- background. On the right is a TEAZ based melanoma created by electroporation of miniCoopR-GFP plus ubb:Cas9 plus zfU6:sgRNA against Rb1. Whereas most of the tumor in the traditional BRAF injected tumor is positive for Rb1 (red staining), the majority of the cells in the TEAZ tumor stain negatively.
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Full text @ Dis. Model. Mech.