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Fig. S1
TEAZ can be used to introduce transgenes in the adult brain and heart. (a) Purified plasmid encoding ubb:GFP (1μl of a 1000 ng/μl solution) was injected through the skull of an anesthetized casper zebrafish directly into the brain cavity using a pulled glass micropipette. The injected zebrafish is then electroporated across the dorsal-ventral axis of the head with the cathode positioned below the jaw and imaged for GFP fluorescence (n=4/5). (b) Pathology of the same electroporated casper zebrafish with hematoxylin and eosin or immunochemistry against GFP to demonstrate reporter expression. Images are visualized at 4x and 20x where scale bars represent 500 μm and 100 μm respectively. (c) TEAZ can be extended to expression within the heart of adult zebrafish. Casper fish were injected into the heart through the gills with 1 μl of 1000ng/μl of a plasmid carrying the myl7:GFP transgene (along with a ubb:Cre cassette that is unrelated for the purposes of this study) (n=2/4). Video of the beating fluorescent heart can be seen in Supplementary Video 1.