ZFIN Image: Mansour et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Jdp2initiates a transplantable T-ALL when expressed from the rag2 promoter. (A) Hematoxylin and eosin staining of peripheral blood and kidney marrow smears from 11-mo-old Tg(rag2:mCherry) and Tg(rag2:jdp2) fish. Note that red blood cells are nucleated in normal zebrafish. Black arrow identifies a mature myeloid cell, and red arrows, circulating blast cells. Bars, 10 µm. (B) Heatmap showing expression of selected genes from FACS-purified cells from normal thymocytes from Tg(rag2:mCherry) zebrafish (each column n = 10 pooled), and tumors from Tg(rag2:jdp2) and Tg(rag2:Myc) fish (each column represents an individual fish), as determined by RNA-seq. RPKM, reads per kilobase of exon per million fragments mapped. (C) mcl1a expression by RNA-seq analysis of FACS-purified fluorescent cells. For the Tg(rag2:Myc) and Tg(rag2:jdp2) genotypes, each data point represents an individual fish, whereas for normal thymus, each point represents pooled cells harvested from 10 Tg(rag2:mCherry) animals. (D) Tumor cells were harvested from Tg(rag2:jdp2) fish and injected into the circulation of 2-d-old recipient fish homozygous for a hypomorphic rag2 allele (E450fs). Two representative embryos are shown, and findings were validated in two independent experiments. Bars, 0.1 mm. (E) Tumor cells harvested from Tg(rag2:jdp2) fish were injected into the peritoneum of adult homozygous rag2^E450fs fish. Recipients were monitored by fluorescence imaging. Findings were validated in three independent experiments. Bars, 0.5 mm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Med.