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Fig. 1
Characterization of a stable eysrmc101/rmc101 zebrafish line.
(A) Sanger sequencing identified a five base pair deletion in exon 20 in eysrmc101/rmc101 zebrafish. (B) Representative gel image of RT-PCR analysis using RNA from a pool of larvae (n = 15), which shows that eys transcripts are present in both wild-type and eysrmc101/rmc101 zebrafish (upper panel). Sanger sequencing confirmed the presence of the five base pair deletion in the eysrmc101/rmc101 transcript (lower panel). (C) Protein domain structures of wild-type Eys and the truncated Eys protein that is predicted in eysrmc101/rmc101 zebrafish.
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