ZFIN Image: Wu et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Il34-Csf1ra Pathway and Neuronal Apoptosis Collaboratively Orchestrate Microglia Colonization

(A and B) Representative images (A) and quantification (B) of microglia in the optic tectum of 3-dpf WT (n = 8), Tg(Xla.Tubb:il34) (n = 6), Tg(Xla.Tubb:bcl-2) (n = 13), and Tg(Xla.Tubb:il34;Xla;Tubb:bcl-2) (n = 5) embryos. Microglia were labeled by Tg(mpeg1:DsRedx) (red). The optic tectum is indicated by dashed lines. Values represent means with SD.

(C and D) WISH showing mfap4 expression (C) and quantification (D) of mfap4⁺ macrophages in the head region of 30-hpf il34^−/− embryos or Tg(Xla.Tubb:bcl-2);il34^−/− embryos. n = 19 and 16 for il34^−/− and Tg(Xla.Tubb:bcl-2);il34^−/− embryos, respectively. Values represent means ± SD.

(E and F) Representative transverse sections through the midbrain and hindbrain of 3-dpf WT or Tg(Xla.Tubb:bcl-2);il34^−/− embryos (E), with macrophages labeled by Tg(mpeg1:DsRedx) (red), neurons labeled by HuC/D immunostaining (green), and quantification of DsRedx⁺ microglia in the whole CNS (F). The CNS region is indicated by dashed lines. n = 6, 11, 6, and 8 for WT, Tg(Xla.Tubb:bcl-2), il34^−/−, and Tg(Xla.Tubb:bcl-2);il34^−/− embryos, respectively. Values represent means with SD.

ns, p > 0.05; ^∗p ≤ 0.05; ^∗∗∗∗p ≤ 0.0001.

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Acknowledgments

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Reprinted from Developmental Cell, 46, Wu, S., Xue, R., Hassan, S., Nguyen, T.M.L., Wang, T., Pan, H., Xu, J., Liu, Q., Zhang, W., Wen, Z., Il34-Csf1r Pathway Regulates the Migration and Colonization of Microglial Precursors, 552-563.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell