Fig. 5

Effect of resveratrol on retinal SIRTs and mitochondrial gene expression after NMDA-induced retinal degeneration. (A) Comparison of retinal morphology of control and treatment groups in the peripheral retina. HE staining of retinal sections are shown. Scale bar: 100 μm. NFL, nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PRL, photoreceptor layer. (B) Quantification of the nerve fiber and GCLs of adult zebrafish retina. Error bars: SEM; n = 5 for HE stained. (unpaired t-test, ***P < 0.001 compared to control) (C, D, F–I) SIRTs, OPA1, and Fis1 genes expression in zebrafish retina after exposure to 50 mg/L resveratrol,100 μM NMDA and 100 μM NMDA with 50 mg/L resveratrol for 24 hours. Graphic representation of average fold changes in (C) SIRT1, (D) OPA1, (F) SIRT3, (G) SIRT4, (H) SIRT5, and (I) Fis1 gene expression by qPCR. Error bars: SEM; n = 4 for qPCR. (unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared to control) (E) Representative Western blot showing the level of SIRT1 and OPA1 protein expression of control and treatment groups for the 24 -hour time point in the retina. Rat served as a positive control; Con, control; RES, 50 mg/L resveratrol; NMDA, 100 μM NMDA; NMDA+ RES, 100 μM NMDA with 50 mg/L resveratrol.