|
Fig. S4
Pronephros repair is independent of Wnt, Celsr1 and Par6 signaling.
(a) In situ hybridization with the pronephros specific marker atp1a1a.4, staining injured zebrafish embryo over-expressing the Wnt inhibitor dkk1. The pronephros was injured 2 days after fertilization and fixed 16 hours later. The injured proximal side (arrow) appears dilated, but recovery was not affected by dkk1 over-expression (Scale bar, 100 μm). (b) Frames from a time-lapse movie of injured transgenic embryo carrying a TCF response element, driving the expression of Red Fluorescence Protein (RFP). Laser ablation caused some background fluorescence (red channel) in the injured cells, but no specific RFP expression was detectable in neighboring cells displaying a migratory response (Scale bar, 10 μm). (c) Quantification of the repair process in HSP:dkk1, celsr1 morpholino oligonucleotide (MO) and par6 MO injected embryos that were injured 2 days after fertilization and fixed 16 hours post wounding. While MO-injected embryos displayed duct abnormalities, repair of the pronephros injury was not affected. (d) Injuries ≤ 50 μm in length were repaired in 100%, injuries ≤ 100 μm, corresponding to 10-12 cell diameters, were repaired in 80%, while no injuries ≤ 150 μm were repaired.