ZFIN Image: Yakulov et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Cilia and fluid flow are not required for pronephros repair

(a) Homozygous ift88(-/-) (ovl) zebrafish embryos were injured 3 days after fertilization, and fixed 2 and 6 hours after wounding. In situ hybridization was performed with the pronephros-specific marker cadherin17 (cdh17). At this stage, the embryos displayed prominent cysts and duct dilatations due to ciliary defects. The gap, clearly visible at 2 hours, was repaired 6 hours after injury. The results were confirmed in three independent experiments with at least 3 control and 3 mutant embryos (Scale bar, 100 μm). (b) Frames from a timelapse movie following the recovery in ift88(-/-) (ovl) embryo. The laser-induced injury was completely repaired over an 8-hour time course (Scale bar, 10 μm).

Acknowledgments

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