ZFIN Image: Moore et al., 2013, Fig. S7

Image

Figure Caption

Fig. S7

Mutation of the HMG box site within Motif3 reduces CNE1 activity. (A) Schematic outlining the mutations introduced into the HMG box site of Motif3. M3Mut1 transgenic zebrafish exhibit a reduction in CNE1 activity compared to controls (compare B to D) (n = 25/41, p<0.001). A similar reduction in CNE1 activity is observed in M3Mut2 transgenics (compare B to G) (n = 6/32, p<0.001). (F) Graphical summary of the effect of HMG binding site mutations upon CNE1 activity in zebrafish embryos. Experiments performed in chick reveal a reduction in CNE1 activity in both M3Mut1 (n = 3/7) and M3Mut2 (n = 2/5) electroporations, however this result did not reach statistical significance (compare C to E and H, respectively).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.