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Fig. S4
Loss of pkd2 expression in zebrafish caused randomization of left–right patterning.
(A) In situ hybridization of pkd2 mRNA in wild-type zebrafish 24 (B) and 48 hpf. (C,D) Left–right asymmetry was visualized by in situ hybridization for cmlc2 to evaluate heart looping. Knockdown of pkd2 expression caused a randomization of heart looping [25]. cmlc2, cardiac myosin light chain 2; hpf, hours post fertilization.
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