ZFIN Image: Roovers et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Tdrd6a Stimulates Buc Mobility in BmN4 Cells

(A) Localization of Buc-eGFP and mCherry-Tdrd6a in BmN4 cells in a single transfection (upper panel) and when they are co-transfected (middle and bottom). Co-transfected BmN4 cells displaying enlarged Buc-eGFP granules to which mCherry-Tdrd6a co-localizes (middle) or dissolved Buc-eGFP (bottom). Scale bars, 10 μm.

(B) Quantification of localization of Tdrd6a transfected 1 day after Buc.

(C) Quantification of localization of Buc transfected 1 day after Tdrd6a.

(D) Co-transfection of Dcp1-mCherry with Buc-eGFP (left) or Dcp1-eGFP with mCherry-Tdrd6a (right). Scale bars, 5 μm.

(E) FRAP recovery curves of mCherry-Tdrd6a and Buc-eGFP (with or without the presence of mCherry-Tdrd6a as indicated). Fluorescence intensity is the calculated fraction of the pre-bleach intensity and is plotted with the 95% confidence interval.

(F) FRAP recovery of Buc-eGFP plotted against increasing relative amounts of mCherry-Tdrd6a present in the bleached granule.

(G) Western blot for GFP and Tdrd6a on transfected BmN4 cell lysates and corresponding pellets.

(H) Pellets of lysates of Buc-EGFP-expressing BmN4 cells in the presence or absence of Tdrd6a as indicated.

See also Figure S6.

Acknowledgments

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Reprinted from Developmental Cell, 46, Roovers, E.F., Kaaij, L.J.T., Redl, S., Bronkhorst, A.W., Wiebrands, K., de Jesus Domingues, A.M., Huang, H.Y., Han, C.T., Riemer, S., Dosch, R., Salvenmoser, W., Grün, D., Butter, F., van Oudenaarden, A., Ketting, R.F., Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification, 285-301.e9, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell