ZFIN Image: Yang et al., 2018, Fig. S9

Image

Figure Caption

Fig. S9

Representation of the zebrafish larvae immersion Infection Model.

(a) The zebrafish microinjection and immersion infection procedures. (b) Generation of caspy2 morphant zebrafish by morpholino oligonucleotide (MO)-mediated knockdown. The upper diagram shows the region of caspy2 targeted to block translation. (c and d) Analysis of the phenotypes of caspy2-MO- and control-MO-injected larvae. (c) Immunoblotting of caspy2 expression in morphants and controls up to 5 days post fertilization (dpf). (d) Representative images of the phenotypes of caspy2-MO- and control-MO-injected zebrafish up to 120 h post fertilization (hpf). (c and d) Results are representative of at least three independent experiments.

Acknowledgments

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