ZFIN Image: Yang et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

Caspy2 plays a critical role in pyroptosis in zebrafish fibroblasts. a–c Wild-type and caspy2-knockout (KO) ZF4 cells were infected with EIB202 or 0909I E. piscicida for 2 h at an multiplicity of infection (MOI) of 50, or left uninfected. a Images were taken as in Fig. 1d. Propidium iodide (PI) was added to detect the loss of plasma membrane integrity. Arrows signify cells with pyroptotic-like features. Scale bar, 50 µm. b Supernatants from the indicated ZF4 cells were analyzed for cell death measured by lactate dehydrogenase (LDH) release. c Mixtures of cell lysates and supernatants were subjected to immunoblotting. (a–c) Results are representative of at least three independent experiments, and error bars denote the SD of triplicate wells. ^*p < 0.05 (t test)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.