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Fig. S5
Validation of epcam-MO. (A-C) Injection of 1.2ng epcam morpholino recapitulated a previously published epcam mutant phenotype. It prevented normal morphogenesis of otoliths (shiny crystals lost- arrows). Co-injection of 30ng rescued this morphant phenotype. (D-G) As in the epcam mutants, there was no obvious lateral line phenotype with injection of 1.2ng epcam morpholino. (G) However, when the dose of epcam morpholino was increased to 5 ng, a more variable number of neuromasts was deposited, consistent with previous studies that had suggested that the epcam morpholinos can have some off-target effects. For our study only the low dose of 1.2 ng was used to minimize potential off target effects. Fig.