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Fig. 5
RA and FA co-supplementation rescue Wnt signaling.
Alcama staining on Tg(Tcf/lef-miniP:dGFP) fish showed that ethanol treatment reduced the number of Wnt+/Alcama+ cells in the peripheral CMZ. Control and ethanol treated embryos in this experiment appeared identical to those shown in Fig 4A–4C and were left out for brevity. (A-F) RA co-supplementation could rescue Wnt+/Alcama+ cells (A-C); FA co-supplementation could also rescue Wnt+/Alcama+ cells in the CMZ particularly in 100 mM Ethanol + FA treated fish (D-F). (G) Quantification of Wnt+/Alcama+ cells in the peripheral CMZ showed significant reduction after ethanol treatment, which was rescued by RA and FA co-supplementation. Numbers of cells were counted in a single optic-nerve containing confocal optical section in the ventral CMZ. Images show rostral at top, lateral at left. Error bars indicate standard deviation. ‘**’ indicates statistical significance in comparison to control embryos (p<0.01). ‘*’ indicates statistical significance in comparison to control embryos (p<0.05). ‘a’ indicates statistical significance in comparison to 100 mM ethanol embryos (p<0.05). ‘b’ indicates statistical significance in comparison to 150 mM ethanol embryos (p<0.05). Scale bar = 10 μm for panels A-F.