ZFIN Image: Zhao et al., 2018, Fig. S1

Image

Figure Caption

Fig. S1

PIK3C3 knockout in zebrafish. (a) Crispr/Cas9 mediated knockout of pik3c3. gRNA targeting sequence in exon 11 is highlighted in red. We recovered two stably transmitted mutant alleles and they are predicted to produce truncated proteins of 394 and 395 aa respectively. (b) qPCR analysis with primers 23F/24R reveals that WT and mutant RNAs are expressed at similar levels. gapdh is the internal control and data represent mean±SD from three independent repeats. NS, non-significant in one-way ANOVA with Dunnett's Multiple Comparison. (c) RT-PCR with the indicated primer pairs generate only one band in each mutant, indicating mutant RNAs are spliced normally. (d) Genotyping results of WT, heterozygote and mutant at the indicated stages. (e) The digestive activities in heterozygous embryos are normal at 8 dpf as revealed by Dextran, PED6 and Enzchek staining. Assays were performed as described in fig.1b.

Acknowledgments

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