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Fig. 4
Subcellular localization of Danio rerio DDX41 (DrDDX41) and identification of the nuclear localization signal motifs within it. (A) Schematic diagram of full-length (FL) and DrDDX41 mutants with the coiled-coil (CC), DEADc, HELICc, ZnF_C2HC domains, and residue numbers as indicated. The various DrDDX41 fragments were inserted into the C-terminus of pEGFP-N1. (B) Representative images of transfected HEK293T cells. The N-terminal 190 amino acids targeted GFP to the nucleus (a,b). Deletion of this region in DrDDX41 (191–613, c) and DrDDX41 (191–411, d) resulting in punctate cytoplasmic staining and exclusion from the nucleus. Scale bars represent 5 µm. Images were captured under a laser-scanning confocal microscope (Zeiss LSM-710; original magnification, 630×).