ZFIN Image: Grajevskaja et al., 2018, Fig. S1

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Figure Caption

Fig. S1

Reversion of tbx5a^tpl58 mutant phenotypes by inverting the gene trap cassette.

A, B. Embryos injected with Flp^o mRNA (A) highly reduced mRFP expression (left) and/or recovery of pectoral fins (right) compared to un-injected siblings (B). C. Screening of R1 (F1) embryos for a reverted allele (tbx5a^tpl58R) by three primer PCR. Embryos in the first three lanes were RFP positive and therefore were expected to carry the non-modified allele (tbx5a^tpl58). Embryos in lanes 4–16 were RFP negative and therefore were expected to be either wild-type (tbx5a⁺) or positive for the inverted allele (tbx5a^tpl58R). D. Fish homozygous for the reverted allele have normal hearts, compared to fish heterozygous for the gene trap. E. Enlarged atrium and blunt, mis-shapen ventricle comparable to the one shown in Fig 2 are indicated by red arrows.

Acknowledgments

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