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Fig. S3
Relative expression patterns of hoxa13a and evx1. (A-L) Double FISH (A-C, E-G, IK) and DAPI counterstains (D, H, L) on longitudinal cryosections of 4dpa fin regenerates. (A-C, E-G, I-K) In joint-forming cells, evx1 and hoxa13a are always co-expressed (yellow arrows). (AH) In presumptive joint cells, hoxa13a is expressed either alone (Pattern I: A-D, green arrowheads) or is co-expressed with evx1 (Pattern II: E-H, yellow arrowheads). (I-K) In Pattern III: hoxa13a and evx1 are co-expressed in joint-forming cells when presumptive joint cells are not present (yellow arrows). (A, E, I) hoxa13a alone. (B, F, J) evx1 alone. (C, G, K) hoxa13a and evx1 expression merged. Scale Bars = 50μm (shown in A). These images are single image views for the merged images in Fig.4A-A”.