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Fig. 5
Proliferating germ cells were reduced, but apoptotic germ cells increased in ar-null zebrafish testes. (A, B) Immunofluorescent staining with anti-vasa antibody identified different types of spermatogenic cells in ar -/- testes and wildtype sibling (ar +/+) testes at 70 dpf or 4.5 mpf, respectively. SG, spermatogonia; SZ, spermatozoa; PSP, primary spermatocyte; SSP, secondary spermatocyte; SPD, spermatid. (C) Immunohistochemistry with anti-cyclin D1 antibody indicated that proliferating germ cells were reduced in ar -/- testes compared with wildtype (ar+/+) siblings at 4.5 mpf. (D) TUNEL assay indicated increased apoptosis of germ cells in ar -/- testes compared with wildtype (ar +/+) siblings at 4.5 mpf. Mpf, months post fertilization. (E, F) Expression of ccnd2a (cyclin d2a) was down-regulated, but expression of gsdf was up-regulated in ar-/- (ar ihb1225/ihb1225) testes compared with wildtype (ar +/+) sibling testes (4.5 mpf, n=3, respectively).