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Fig. 3
HDACs have opposing effects on primary cilia. A–D: Lateral views of 72‐hpf embryos immunostained for acetylated tubulin (green) reveal cilia at the junction of a pronephric duct with the cloaca. Scale bar = 5 μm. Cloacal cilia number and length were assessed at 72 hpf (E,F). n = 15–30 embryos. *P < 0.005 in comparison to controls. Cardiac asymmetry was analyzed by cmlc2 expression at 24 hpf (G–I) and plotted as a percentage of embryos (J). n = 20–38 embryos. Primary cilia length in the KV at the 12‐somite stage (K–M) is averaged (N). n = 40–60 cilia. Scale bar = 5 μm. *P < 0.005.
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