Fig. S5

Fig. S5

Azumolene reduces ectopic muscle allocation and DRG formation caused by overactivation of the Shh signaling pathway. Related to Figure 6. (A) Quantification of Shh-dependent muscle cell types (as determined by transcription factor expression in nuclei) in embryos with experimentally activated Shh signaling. Data are derived from experiments illustrated in Figures 6F-6M. For each condition, the number of embryos analyzed is indicated. Fold change in the number of nuclei was calculated relative to 0.5% DMSO-treated controls. Data are represented as mean ±SEM. *p<0.01; ***p<0.0001; n.s., not significant. (B-D) Mutants that fail to express functional Patched2 receptor have an overproduction of DRGs, which is reduced by treatment with azumolene. Pan-neuronal staining with anti-HuC was used to mark DRGs (white arrowheads). (C) MZptch2^(-/-) mutants have an increased number of DRGs, some of which form in ectopic locales (yellow arrowheads). (D) DRG formation in MZptch2^(-/-) embryos is eliminated by azumolene treatment. (E) Quantification of Figures S5B-S5D. Each point represents a single embryo and the line indicates the mean. *p<0.01; ***p<0.0001.