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Fig. S7
A-H. Validation of the efficiency and specificity of the hey1 morpholinos. Whole-mount in situ hybridization for the expression of pomca (A-D) and gh (E-H) in 72hpf larvae, wild-type (A,E) or injected at the one-cell stage with the MOs indicated (B-D, F-H). High magnification of the pituitary area. I. Quantification of the number of GH-positive neurons in embryos as in panels E-H. n=7 embryo for each condition. **: p=0.006; ***: p<0.0001. J-N. RG proliferation is unaffected in hey1-/- larvae. Cross-sections of the pallium in wildtype (K,K’) and hey1- /- (L,L’) 7dpf larvae, immunoprocessed for Gs and Pcna (K,L) or Gs and BrdU (K’,L’) following a short BrdU pulse, and corresponding quantification of the proportion of aRG (M) or BrdU-positive aRG (N) among the whole RG population. n=4 larvae and 250 RG cells counted for each genotype. There was no Cas3-positive cell among RG cells. Scale bars: A-D: 20μm, E-H: 10 μm, K-L’: 20 μm.