Fig. 6
The FMA acts as a calcium-dependent modified midbody. (A-D) EMTB::EGFP transgenic fish. In wild type (A), the FMA initially aligns along the furrow (brackets) and becomes enriched distally (arrowheads). In nebel mutants (B), the FMA is initially normal but bundles in medial furrow regions. In 2-APB-treated wild-type embryos (C), the FMA forms normally but remains aligned along the furrow. During the early cleavage stages of wild-type EMTB::EGFP embryos (D), the FMA forms (bracket) but bundles medially (arrow). Time is in min. (E) Fixed wild-type 64-cell embryos stained for microtubules and for DNA (DAPI), showing inwardly bundling FMAs (arrowhead) and remnants (arrow). Scale bar: 75 μm in E.