ZFIN Image: Hiscock et al., 2018, Fig. S3

Image

Figure Caption

Fig. S3

A: Left: We compare regions adjacent to a mitotic cell (15 μm x 15 μm apical surface, manually contoured) [orange], to nearby control regions of the same dimensions but without an arrested cell [cyan]. Single cell tracking reveals that there is little movement of cells along AP/DV that would take them out of the regions (~2.6μm mean distance moved). Right: segmented image. Asterisks depict an arrested mitotic cell.

B: Illustrative calculation. We define the fraction of neurons as , i.e. for the right . We define the proliferation rate between the two timepoints as: .

(C) There is an increased fraction of neurons adjacent to arrested mitotic cells (red) compared to control regions without an arrested cell (blue) (p < 0.01). Proliferation rates are similar in the two cases (right) (p = 0.9). (n = 7 for both cases)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development