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Figure Caption
Fig. 1-S1
Beta-cell proliferation declines with age in secondary islets.
(a,b) Confocal projection of whole-mount secondary islets from Tg(ins:Fucci-G1);Tg(ins:Fucci-S/G2/M) animals at 35 dpf, 3 mpf and 1 ypf. Scale bar 20 µm. (c) Quantification of percentage of Tg(ins:Fucci-S/G2/M)-positive and Tg(ins:Fucci-G1)-negative (green-only) beta-cells from 35 dpf (n = 8 fish, secondary islets = 16), 3 mpf (n = 8 fish, secondary islets = 70) and 1 ypf (n = 8 fish, secondary islets = 76) animals. Error bars show s.e.m. (one-way ANOVA, *p<0.05).
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