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Fig. S4
Whole mount in situ hybridization experiments for mmp genes
qPCR results shown in Fig. 3M-3O were validated with the aid of in situ hybridization experiments for (A) mmp9, (B) mmp13a, and (C) mmp14b at 7 dpf. Increased expression of mmp13a (indicated by an arrow), the most highly expressed gene among these three, was confirmed. We could not detect expression of the other two genes in our hands probably due to lower expression levels. Shown are the representative images chosen from approximately 10 larvae. Scale bar indicates 500 m.
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