ZFIN Image: Enya et al., 2018, Fig. 1

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Figure Caption

Fig. 1

Screening for Gal4 lines that induce outgrowth of target organs when crossed with Tg(5×UAS:EGFP-P2A-kras^G12D). (A) The structure of 5×UAS:EGFP-P2A-kras^G12D. The gray triangles represent the sequence recognized by Tol2 transposases. (B-Q) Screening for a Gal4 line that is potent to induce outgrowth of target organs. Images of the sibling control (left) and EGFP-kras^G12D-expressing larvae (right) are shown. Higher-magnification images are also presented. Images on the left for each group are bright-field images, whereas the others are fluorescence images (EGFP). Target organs are outlined by a dashed white line for (B-E) gSAIzGFFD1105A (pInt-Gal4) (7 dpf), (F-I) gSAIzGFFM103B (aInt-Gal4) (7 dpf), (J-M) gSAIzGFFD886A (Liver-Gal4) (7 dpf) and (N-Q) gSAGFF138A (Brain-Gal4) (3 dpf). Larvae without EGFP expression from the same clutch were used as sibling controls. White arrows indicate organs that express the EGFP-kras^G12D transgene. Scale bar: 1 mm. Data are representative of at least two independent experiments.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.