ZFIN Image: Shibata et al., 2018, Fig. 3

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Figure Caption

Fig. 3

Early RE cells are disposed of by apoptosis by 5 dpa during zebrafish fin regeneration. (A) Tracking of early RE cells that were pulse-labelled at 9-10 dpa with 0.1 µM 4-OH TAM. The labelled cells moved distally and disappeared by 5 dpa. The longitudinal section of the 2 dpa fin shows that the EGFP⁺ cells localize to the tip of the wound epidermis. The dotted lines indicate the amputation planes. Scale bars: 200 µm (fins) and 50 µm (section). (B) Quantification of the number of EGFP⁺ cells in A. EGFP⁺ cells in three adjacent rays were counted (n=4 zebrafish). (C) Detection of the TUNEL⁺ cells (red) and EGFP⁺ cells derived from the early RE cells that were labelled by Cre-loxP recombination at 9-10 hpa. The arrowheads show the TUNEL⁺ and EGFP⁺ cells. Scale bar: 50 µm.

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