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Fig. 6
Knockdown (KD) of Dachd in zebrafish larvae affected podocyte gene expression and induced foot process effacement. A, Cryosections of 3 dpf zebrafish larvae showed an increase in Bowman's space (arrows) after the KD of Dachd (n = 3). Nephrin staining is shown in the middle panel. Scale bar represents 20 μm. B, Electron microscopy of Dachd KD and Ctrl morpholinos (MO) zebrafish larvae. Ctrl MO larvae showed a normally structured filtration barrier with intact foot processes (arrows). Foot processes of dachd vivo MO larvae were disordered with microvilli‐like structures (arrowheads) protruding in an increased Bowman's space (Bo). glomerular basement membrane and endothelial cells were also affected by MO treatment (circle). Scale bars represent 10 μm in the upper and 1 μm in the lower pictures. P, podocyte; E, endothelial cell; Ca, capillary. C, The expression of podocyte genes (synpo, nphs1 and nphs2) was determined by qRT‐PCR analysis. Total mRNA extracts of whole larvae (3 dpf) injected with Ctrl MO (white bars, means ± SD, n = 5), dachd splice MO (gray bars, means ± SD, n = 5) or dachd trans MO (black bars, means ± SD, n = 5) were used for cDNA synthesis. Data sets were normalized to Ctrl MO‐injected samples and the reference genes rpl13, eef1a1l1 and 18sRNA. ****P < .0001, ***P < .001, **P < .01, *P < .05
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Full text @ J. Cell. Mol. Med.