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Fig. 4
adamts16-morphant OCs proliferate slowly, and exhibit increased apoptosis activity. (A, B) Images of 36-hpf zebrafish eye sagittal sections stained with anti-BrdU antibody (red). (C) Percentage quantification of BrdU+ cells/eye in control and MO1- and MO2-morphant groups. (D, E) TUNEL staining (green) of 36-hpf zebrafish eye sagittal sections. (F) Quantification of TUNEL+ cells/section in control, MO1-, and MO2-morphant groups. (G) Quantification of coloboma prevalence shows that the coloboma phenotype in MO1- or MO2-morphants could not be rescued by p53 MO knockdown. Numbers under each column denote the number of zebrafish injected in each group. Scale bars in (B) and (E): 50 μm.
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Full text @ Invest. Ophthalmol. Vis. Sci.