ZFIN Image: Liu et al., 2018, Fig. S5

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Figure Caption

Fig. S5

Hematopoietic defect in apoc2 mutants is not due to delayed angiogenesis. (A) Angiogenesis in fli1:eGFP WT, apoc2 mutant, the apoc2 mutant synchronized with WT, and the apoc2 mutant injected with DHA, all imaged at 80 hpf. apoc2 mutant synchronization was achieved by maintaining the embryos at 30°C from 72 to 80 hpf, while WT embryos were kept at 28°C. DHA was injected at 48 hpf. Yellow arrows point to sub-intestinal vessels. Red arrows point to the yolk. (B) In situ hybridization with runx1/cmyb primers in the same groups as in panel A, at 80 hpf. The CHT regions traced with red dashed quadrangles are enlarged in right-hand panels. Scale bars, 200 μm.

Acknowledgments

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